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Enhanced succinic acid production in Corynebacterium glutamicum with increasing the available NADH supply and glucose consumption rate by decreasing H+-ATPase activity
- Xu, Hongtao, Zhou, Zhihui, Wang, Chen, Chen, Zhongjun, Cai, Heng
- Biotechnology letters 2016 v.38 no.7 pp. 1181-1186
- Corynebacterium glutamicum, H-transporting ATP synthase, NAD (coenzyme), bioreactors, byproducts, fermentation, glucose, glycolysis, mutants, point mutation, succinic acid
- OBJECTIVES: To enhance succinic acid production in Corynebacterium glutamicum by increasing the supply of NADH and the rate of glucose consumption by decreasing H⁺-ATPase activity. RESULTS: A mutant of C. glutamicum NC-3-1 with decreased H⁺-ATPase activity was constructed. This increased the rate of glycolysis and the supply of NADH. Fermentation of C. glutamicum NC-3-1 gave 39 % higher succinic acid production (113 and 81 g/l), a 29 % higher succinic acid yield (0.94 and 0.73 g succinic acid/g glucose) and decreased by-products formation compared to that of C. glutamicum NC-3 in 5 l bioreactor. CONCLUSION: The point mutation in C. glutamicum NC-3-1 increased the rate of glycolysis and resulted in higher succinic acid production, higher succinic acid yield and significantly decreased formation of by-products.