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New Source of Genetic Polymorphisms in Lepidoptera?
- Hundsdoerfer, Anna K., Wink, Michael
- Zeitschrift für Naturforschung 2014 v.60 no.7-8 pp. 618-624
- DNA fingerprinting, Pieridae, Pyralidae, Sphingidae, butterflies, data analysis, genetic polymorphism, genome, microsatellite repeats
- The variability level of the ISSR (inter-simple sequences repeat) primer (GACA)₄ was examined in the three Lepidoptera families Pyralidae, Sphingidae and Pieridae. Our study shows that the tetra-repeat (GACA)ₙ is evidently present in sufficient numbers in these butterflies to provide informative DNA fingerprints. The variability is mostly rather high, but within a comparable range to other ISSR studies. Although less polymorphisms may be encountered in some butterfly families, this study indicates that high variability of this marker may be a common characteristic of Lepidoptera genomes. An appeal for a minimal level of standardization of ISSR-PCR data analysis is formulated to enable an exact comparison between the groups of organisms studied with this fingerprint technique.