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Optimized Nutrient Medium for Galanthamine Production in Leucojum aestivum L. in vitro Shoot System

Georgiev, Vasil, Berkov, Strahil, Georgiev, Milen, Burrus, Monique, Codina, Carles, Bastida, Jaume, Ilieva, Mladenka, Pavlov, Atanas
Zeitschrift für Naturforschung 2014 v.64 no.3-4 pp. 219-224
Leucojum aestivum, alkaloids, ammonium nitrate, ammonium sulfate, biosynthesis, potassium dihydrogen phosphate, potassium nitrate, regression analysis, sucrose
The common effect of NH₄⁺, NO₃⁻, KH₂PO₄ and sucrose on the biosynthesis of galanthamine by a Leucojum aestivum shoot culture was studied. Polynominal regression models were elaborated for the description of the galanthamine biosynthesis as a consequence of variation of the investigated variables (NH₄⁺ between 0.20 and 0.54 g/L; NO₃⁻ between 1.44 and 3.44 g/L; KH₂PO₄ between 0.10 and 0.24 g/L, and sucrose between 30.00 and 60.00 g/L). Optimization procedures allowed us to establish the optimal concentrations of the investigated variables and to propose the modified MS nutrient medium, with 4.50 g/L KNO₃, 0.89 g/L NH₄NO₃, 1.25 g/L (NH₄)₂SO₄, 0.10 g/L KH₂PO₄ and 60 g/L sucrose, for the galanthamine production by a Leucojum aestivum shoot culture. The proposed modified MS medium provided considerable increase of both the production yield and the relative content of the target alkaloid in the alkaloid mixture.