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Optimized Nutrient Medium for Galanthamine Production in Leucojum aestivum L. in vitro Shoot System
- Vasil Georgiev, Strahil Berkov, Milen Georgiev, Monique Burrus, Carles Codina, Jaume Bastida, Mladenka Ilieva, Atanas Pavlov
- Zeitschrift für Naturforschung 2014 v.64 no.3-4 pp. 219-224
- Leucojum aestivum, alkaloids, ammonium nitrate, ammonium sulfate, biosynthesis, potassium dihydrogen phosphate, potassium nitrate, regression analysis, sucrose
- The common effect of NH₄⁺, NO₃⁻, KH₂PO₄ and sucrose on the biosynthesis of galanthamine by a Leucojum aestivum shoot culture was studied. Polynominal regression models were elaborated for the description of the galanthamine biosynthesis as a consequence of variation of the investigated variables (NH₄⁺ between 0.20 and 0.54 g/L; NO₃⁻ between 1.44 and 3.44 g/L; KH₂PO₄ between 0.10 and 0.24 g/L, and sucrose between 30.00 and 60.00 g/L). Optimization procedures allowed us to establish the optimal concentrations of the investigated variables and to propose the modified MS nutrient medium, with 4.50 g/L KNO₃, 0.89 g/L NH₄NO₃, 1.25 g/L (NH₄)₂SO₄, 0.10 g/L KH₂PO₄ and 60 g/L sucrose, for the galanthamine production by a Leucojum aestivum shoot culture. The proposed modified MS medium provided considerable increase of both the production yield and the relative content of the target alkaloid in the alkaloid mixture.