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Assessment of Enzyme Inhibitory and Antioxidant Activities of Lignans from Taxus baccata L.

Author:
Nurgun Kucukboyacı, Ilkay Orhan, Bilge Şener, S. Ahmad Nawaz, M. Iqbal Choudhary
Source:
Zeitschrift für Naturforschung 2014 v.65 no.3-4 pp. 187-194
ISSN:
1865-7125
Subject:
Alzheimer disease, acetylcholinesterase, ions, Taxus baccata, 2,2-diphenyl-1-picrylhydrazyl, iron, linoleate 13S-lipoxygenase, chromatography, pathogenesis, lignans, cholinesterase, chlorogenic acid, antioxidant activity
Abstract:
Phytochemical investigations of Taxus baccata L. by successive chromatographic methods resulted in the isolation of the lignans lariciresinol (1), taxiresinol (2), 3´-demethylisolariciresinol- 9´-hydroxyisopropylether (3), isolariciresinol (4), and 3-demethylisolariciresinol (5) as well as taxoids. Compounds 1 - 5 were evaluated for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and lipoxygenase (LOX) inhibitory activities, which play a role in the pathogenesis of Alzheimer’s disease (AD), by in vitro spectrophotometric methods, while they were also screened for their antioxidant capacity in 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging, ferrous ion-chelating effect, and ferric-reducing antioxidant power (FRAP) at 125, 250, 500, and 1000 μg ml-1. All compounds exhibited a moderate inhibition against both BChE and LOX, whereas they were inactive towards AChE. The compounds displayed a great scavenging activity against DPPH especially at 500 and 1000 μg ml⁻¹. Besides, they were found to exert noteworthy reducing antioxidant power on ferric ions. In particular, the FRAP of compounds 2 (3.552 ± 0.02), 4 (3.021 ± 0.71), and 5 (3.533 ± 0.01) were as high as that of the reference chlorogenic acid (3.618 ± 0.01) at 1000 μg ml⁻¹. None of the compounds exhibited chelating ability against ferrous ions.
Agid:
5231311