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Induction of Caspase-8 and Death Receptors by a New Dammarane Skeleton from the Dried Fruits of Forsythia koreana

Hawas, Usama W., Gamal-Eldeen, Amira M., El-Desouky, Samy K., Kim, Young-Kyoon, Huefner, Antje, Saf, Robert
Zeitschrift für Naturforschung 2014 v.68 no.1-2 pp. 29-38
DNA fragmentation, Forsythia viridissima var. koreana, apoptosis, breast neoplasms, caffeic acid, caspase-8, cations, cytotoxicity, death domain receptors, dried fruit, fruits, humans, necrosis, neoplasm cells, nuclear magnetic resonance spectroscopy, rosmarinic acid, rutin, skeleton, spectral analysis, ursolic acid
A new naturally occurring compound based on the dammarane skeleton, i.e. cabralealactone 3-acetate-24-methyl ether, was isolated from the aqueous methanolic extract of Forsythia koreana fruits, along with eight known compounds: cabralealactone 3-acetate, ursolic acid, arctigenin, arctiin, phillyrin, rutin, caffeic acid, and rosmarinic acid. The identifi cation of the isolated compounds was based on their spectral analysis including: HREI-MS, 1D and 2D NMR spectroscopy. The selected compounds and the aqueous methanolic extract were evaluated for their cytotoxic activity against human solid tumour cell lines. Cabralealactone 3-acetate-24-methyl ether and ursolic acid were found to be active against human breast cancer cells (MCF-7). The cytotoxicity was associated with the activation of caspase-8, the induction of the death receptors DR4 and DR5, as well as DNA fragmentation, and was thus due to apoptosis rather than necrosis