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A high-resolution comparative map of porcine chromosome 4 (SSC4)

Author:
Ma, J.-G., Chang, T.-C., Yasue, H., Farmer, A.D., Crow, J.A., Eyer, K., Hiraiwa, H., Shimogiri, T., Meyers, S.N., Beever, J.E., Schook, L.B., Retzel, E.F., Beattie, C.W., Liu, W.-S.
Source:
Animal genetics 2011 v.42 no.4 pp. 440-444
ISSN:
0268-9146
Subject:
bacterial artificial chromosomes, humans, hybrids, loci, microsatellite repeats, swine, transcriptome
Abstract:
We used the IMNpRH2₁₂ ₀₀₀-rad RH and IMpRH₇ ₀₀₀-rad panels to integrate 2019 transcriptome (RNA-seq)-generated contigs with markers from the porcine genetic and radiation hybrid (RH) maps and bacterial artificial chromosome finger-printed contigs, into 1) parallel framework maps (LOD ≥ 10) on both panels for swine chromosome (SSC) 4, and 2) a high-resolution comparative map of SSC4, thus and human chromosomes (HSA) 1 and 8. A total of 573 loci were anchored and ordered on SSC4 closing gaps identified in the porcine sequence assembly Sscrofa9. Alignment of the SSC4 RH with the genetic map identified five microsatellites incorrectly mapped around the centromeric region in the genetic map. Further alignment of the RH and comparative maps with the genome sequence identified four additional regions of discrepancy that are also suggestive of errors in assembly, three of which were resolved through conserved synteny with blocks on HSA1 and HSA8.
Agid:
523224