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Determination of nine volatile N‐nitrosamines in tobacco and smokeless tobacco products by dispersive solid‐phase extraction with gas chromatography and tandem mass spectrometry

Lv, Fang, Guo, Junwei, Yu, Fei, Zhang, Tingting, Zhang, Shimin, Cui, Huapeng, Liu, Xianjun, Chen, Li, Liu, Leiyu, Liu, Shaofeng, Xie, Fuwei
Journal of separation science 2016 v.39 no.11 pp. 2123-2128
N-nitrosodimethylamine, carbon, detection limit, diethylnitrosamine, gas chromatography, smokeless tobacco, solid phase extraction, tandem mass spectrometry
A method was developed for the determination of nine volatile N‐nitrosamines in tobacco and smokeless tobacco products. The targets are N‐nitrosodimethylamine, N‐nitrosopyrrolidine, N‐nitrosopiperidine, N‐nitrosomorpholine, N‐nitrosoethylmethylamine, N‐nitrosodiethylamine, N‐nitrosodipropylamine, N‐nitrosobuylmethylmine, and N‐nitrosodibutylamine. The samples were treated by dispersive solid‐phase extraction using 1 g of primary secondary amine and 0.5 g of carbon and then analyzed by gas chromatography with tandem mass spectrometry with an electron impact ion source. The recoveries for the targets ranged from 84 to 118%, with <16% relative standard deviations at three spiking levels of 0.5, 1.25, and 2.5 ng/g. The limits of detection ranged from 0.03 to 0.15 ng/g. With the use of the proposed method, we detected the presence of six nitrosamines in the range of 0.4–30.7 ng/g. The study demonstrated that the method could be used as a rapid, convenient, and high‐throughput method for N‐nitrosamines analysis in tobacco matrix.