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Use of quantitative PCR with the chloroplast gene rps4 to determine moss abundance in the early succession stage of biological soil crusts
- Deng, Songqiang, Wang, Chunzi, De Philippis, Roberto, Zhou, Xiangjun, Ye, Chaoran, Chen, Lanzhou
- Biology and fertility of soils 2016 v.52 no.5 pp. 595-599
- biomass, chlorophyll, chloroplasts, community structure, fungi, genes, mosses and liverworts, quantitative polymerase chain reaction, ribosomal RNA, soil crusts, China
- The quantitative PCR (qPCR) method was firstly used to measure moss abundance with moss chloroplast gene rps4 as compared to that with cyanobacterial 16S rRNA gene and fungal 25-28S rRNA gene in the early succession stage of biological soil crusts (BSCs). Four sites with three BSC types collected from Hobq Desert of China, representing cyanobacterial-, lichen-, and moss-dominated BSCs were investigated. The copies of the moss rps4 gene, cyanobacterial 16S rRNA gene, and fungal 25-28S rRNA gene, chlorophyll a content, and the community composition variated significantly. The moss rps4 gene copies attained a significant positive correlation with chlorophyll a content and showed a cross-validation with relative moss biomass.