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Purification and characterization of a surfactant-compatible lipase from Aspergillus tamarii JGIF06 exhibiting energy-efficient removal of oil stains from polycotton fabric

Author:
Das, Arijit, Shivakumar, Srividya, Bhattacharya, Sourav, Shakya, Sujina, Swathi, S.S.
Source:
3 Biotech 2016 v.6 no.2 pp. 131
ISSN:
2190-572X
Subject:
Aspergillus tamarii, agarose, ammonium chloride, barium, beta-mercaptoethanol, bromides, calcium, carboxylic ester hydrolases, chloroform, chromatography, coconut oil, cold, detergents, energy efficiency, enzyme activity, ion exchange, metal ions, molecular weight, olive oil, pH, peanut oil, polyacrylamide gel electrophoresis, polysorbates, sodium dodecyl sulfate, solvents, stain removal, submerged fermentation, surfactants, tryptones, vegetable oil, water
Abstract:
An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimal lipase activity was recorded at pH 4 and 37 °C. The enzyme revealed broad specificity towards different vegetable oils. The K ₘ and V ₘₐₓ of the lipase on olive oil was found to be 330.4 mg and 53,690 U/ml/min, respectively. The lipase activity was stable in the presence of surfactants such as cetrimonium bromide, sodium dodecyl sulphate and Tween 80, and metal ions and reagents such as Ca²⁺, Ba²⁺ and 2-mercaptoethanol. However, the activity was greatly reduced in the presence of organic solvents such as chloroform. The stain removal potential of the crude lipase was determined on polycotton fabric pieces stained with peanut oil. Lipase added to cold water alone significantly enhanced the removal of stain by 152 %. The addition of lipase also improved the stain removal efficiency of a commercially available detergent in the presence of either cold (25 ± 2 °C) or hot (65 ± 2 °C) water. The current findings suggest the potentiality of this enzyme for energy-efficient biocatalytic application.
Agid:
5233116