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Proanthocyanidins, Isolated from Choerospondias axillaris Fruit Peels, Exhibit Potent Antioxidant Activities in Vitro and a Novel Anti-angiogenic Property in Vitro and in Vivo

Author:
Li, Qian, Wang, Xieyi, Dai, Taotao, Liu, Chengmei, Li, Ti, McClements, David Julian, Chen, Jun, Liu, Jiyan
Source:
Journal of agricultural and food chemistry 2016 v.64 no.18 pp. 3546-3556
ISSN:
0021-8561
Subject:
Danio rerio, angiogenesis, antioxidant activity, endothelial cells, free radical scavengers, fruit peels, genetically modified organisms, high performance liquid chromatography, humans, mass spectrometry, median effective concentration, mitogen-activated protein kinase, neoplasms, phosphorylation, proanthocyanidins, signal transduction, toxicity, umbilical veins
Abstract:
The production of new blood vessels (angiogenesis) is an important stage in the growth and spread of cancerous tumors. Anti-angiogenesis is one strategy for controlling tumor progression. This study evaluated the antioxidant and anti-angiogenic activities of a proanthocyanidins (PAs) extract from Choerospondias axillaris peels. HPLC-MS analysis revealed that numerous oligomeric forms of the PAs were detected in the PAs extract, including dimers, trimers, tetramers, and flavan-3-ol monomers. The PAs extract possessed appreciable free radical scavenging activity (IC50/DPPH = 164 ± 7 μg/mL, IC50/ABTS = 154 ± 6 μg/mL), potent reducing power (0.930 ± 0.030 g AAE/g), and strong cellular antioxidant activity (EC50 = 10.2 ± 1.4 and 38.9 ± 2.1 μg/mL without or with PBS-wash, respectively). It could also retard various stages of angiogenesis, such as the migration of endothelial cells and the creation of tubes, without causing toxicity to the cells. With regard to intracellular signal transduction, the PAs extract attenuated the phosphorylation of Akt, ERK, and p38MAPK dose-dependently in endothelial cells from human umbilical veins. In transgenic zebrafish embryo, new blood vessel formation was suppressed by PAs extract in a concentration-dependent manner at 72 h post fertilization. Thus, these results suggest that PAs from C. axillaris peels could be a good source of natural inhibitors to target angiogenesis.
Agid:
5234244