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Antiallergic Activity of Ethanol Extracts of Arctium lappa L. Undried Roots and Its Active Compound, Oleamide, in Regulating FcεRI-Mediated and MAPK Signaling in RBL-2H3 Cells

Author:
Yan, Woong-Suk, Lee, Sung Ryul, Jeong, Yong Joon, Park, Dae Won, Cho, Young Mi, Joo, Hae Mi, Kim, Inhye, Seu, Young-Bae, Sohn, Eun-Hwa, Kang, Se Chan
Source:
Journal of agricultural and food chemistry 2016 v.64 no.18 pp. 3564-3573
ISSN:
0021-8561
Subject:
Arctium lappa, acetates, active ingredients, anaphylaxis, ethanol, histamine, interleukin-4, mice, mitogen-activated protein kinase, rats, roots, secretion, tumor necrosis factor-alpha
Abstract:
The antiallergic potential of Arctium lappa L. was investigated in Sprague–Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) of A. lappa (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined by the level of β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) in rats and attenuated anaphylaxis and histamine release in mice. To identify the active compound of ALE, we subsequently fractionated and determined the level of β-hexosaminidase in all subfractions. Oleamide was identified as an active compound of ALE, which attenuated the secretion of histamine and the production of tumor necrosis factor (TNF)-α and interleukin-4 (IL-4) in cells treated with compound 48/80 or A23187/phorbol myristate acetate (PMA). Oleamide suppressed FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), and p38 mitogen-activated protein kinases (p38-MAPKs). These results showed that ALE and oleamide attenuated allergic reactions and should serve as a platform to search for compounds with antiallergic activity.
Agid:
5234246