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Identification of the target region including the Foc0 1 /foc0 1 gene and development of near isogenic lines for resistance to Fusarium Wilt race 0 in chickpea
- Jendoubi, W., Bouhadida, M., Millan, T., Kharrat, M., Gil, J., Rubio, J., Madrid, E.
- Euphytica 2016 v.210 no.1 pp. 119-133
- Cicer arietinum, Fusarium oxysporum, Fusarium wilt, chickpeas, chromosome mapping, disease resistance, genes, isogenic lines, linkage groups, plant breeding, resistance mechanisms
- Three recombinant inbred populations (RIP-1, RIP-7 and RIP-10) were used to validate and saturate the target area involved in the resistance to Fusarium oxysporum f.sp. ciceris race 0 (Foc0) in chickpeas. With the aim of physically localizing the genetic area controlling Foc0 resistance, a new genetic map of the linkage group 5 (LG5) and a physical map of the Ca5 pseudomolecule were drawn up and compared. The genetic and physical between CaGM20820 and CaGM20889 (16759541–17501349 bp) within an interval of 2 cM comprising 27 annotated genes. Only two of the annotated genes in this region could mapping results obtained were useful to refine the position of the target area be involved in resistance mechanisms. The three RIPs were used to develop three pairs of near isogenic lines (NILs) for resistance/susceptibility to Foc0. NILs are a valuable tool for fine mapping, refining the target region and selecting candidate genes for resistance to Foc0. The information reported in this paper will help develop diagnostic markers, considered useful tools for MAS in chickpea breeding programs.