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Fate of Free and Conjugated Mycotoxins within the Production of Distiller’s Dried Grains with Solubles (DDGS)

Dzuman, Zbynek, Stranska-Zachariasova, Milena, Vaclavikova, Marta, Tomaniova, Monkia, Veprikova, Zdenka, Slavikova, Petra, Hajslova, Jana
Journal of agricultural and food chemistry 2016 v.64 no.24 pp. 5085-5092
animal health, corn, deoxynivalenol, drying, enzymes, feed contamination, fermentation, financial economics, fumonisin B1, glycosylation, tandem mass spectrometry, ultra-performance liquid chromatography, yeasts
Contamination of feed with mycotoxins represents a serious worldwide problem concerning animal health and related economic losses. The present paper provides comprehensive knowledge about the fate of mycotoxins during the production of distiller’s dried grains with solubles (DDGS). The study was carried out using naturally infected maize material in five repetitions. For mycotoxin analysis, a QuEChERS-like (“Quick, Easy, Cheap, Effective, Rugged, and Safe”) isolation approach and ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used. A significant increase of deoxynivalenol (DON) and its glycosylated form, DON-3-glucoside (DON-3-Glc), was observed during the first part of fermentation, when hydrolytic enzymes were added. After yeast addition, the total DON content rapidly decreased. An opposite trend was observed for fumonisin B1 (FB1), in which yeast addition contributed to increase of its content. Further considerable change in mycotoxin content occurred during the drying step, in which approximately two-thirds of the original content was lost.