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Molecular cloning, expression analysis and subcellular localization of LEAFY in carrot (Daucus carota L.)

Zhang, Chunsha, Zhang, Hongwei, Zhan, Zongxiang, Liang, Yi
Molecular breeding 2016 v.36 no.7 pp. 89
Allium cepa, Daucus carota, Ziziphus, amino acids, carrots, complementary DNA, flowering, fluorescence, genes, inflorescences, leaves, meristems, molecular cloning, onions, open reading frames, quantitative polymerase chain reaction, transcription factors
FLO/LEAFY (LFY) genes in the FLO_LFY superfamily are important factors regulating plant transition from inflorescence to floral meristems. In this study, we cloned a full-length LFY cDNA from leaves of carrot (Daucus carota L.). The sequenced gene, designated as DcLFY (GenBank accession number KU297945), comprised 1368 with a 1176-bp open reading frame. The deduced encoded protein, which contained 391 amino acid residues and possessed typical N- and C-domains, shared 67.82 % similarity with LFY of Ziziphus jujube. Quantitative real-time PCR analysis revealed that the relative expression of DcLFY in carrot was the highest in inflorescences and gradually decreased with the growth of the inflorescence during flowering. Expression of green fluorescent protein-fused DcLFY in onion (Allium cepa L.) epidermal cells was localized in nuclei. These results demonstrate that DcLFY is a putative FLO/LFY homolog related to the flowering pathway. Taken together, our data suggest that DcLFY is a crucial transcription factor in floral initiation and floral development in carrot.