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Paradonghicola geojensis gen. nov., sp. nov., isolated from seawater, Geoje-si, South Korea

Lee, Kalam, Srinivasan, Sathiyaraj, Lee, Sang-Seob
Archives of microbiology 2016 v.198 no.6 pp. 501-507
DNA, Sulfitobacter pontiacus, aerobes, bacteria, catalase, chemotaxonomy, fatty acids, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, ribosomal RNA, seawater, sequence homology, sodium chloride, ubiquinones, South Korea
A Gram-negative, non-motile, non-spore-forming and aerobic bacterium, designated FJ12ᵀ, was isolated from seawater, Geoje-si, South Korea. Strain FJ12ᵀ was catalase and oxidase positive. Growth of strain FJ12ᵀ was optimally observed at 30 °C and pH 6 and grew in the presence of 0–11 % (w/v) NaCl (optimum 2–3 % NaCl). The phylogenic analysis based on 16S rRNA gene sequence of strains FJ12ᵀ showed the highest sequence similarity to those of Doghicola eburneus KCTC 12735ᵀ (95.4 %), Aestuariihabitans beolgyonensis KTCT 32324ᵀ (95.3 %), Sulfitobacter pontiacus KCTC 32185ᵀ (94.9 %), Roseisalinus antarcticus DSM 11466ᵀ (94.8 %) and Loktanella soesokkaensis KCTC 32425ᵀ (94.7 %). The major polar lipids of FJ12ᵀ were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid and unidentified lipids. It contained Q-10 as the predominant ubiquinone, and major fatty acid is C₁₈:₁ ω7c (51.7 %), C₁₆:₀ (24.0 %). The DNA G+C content of the strain FJ12ᵀ was 60 mol%. Based on physiological and chemotaxonomic characteristics, strain FJ12ᵀ should be regarded as the type species of a novel genus in the family Rhodobacteraceae for which the name Paradonghicola geojensis sp. nov. is proposed. The type strain FJ12ᵀ (=KEMB 3001-336ᵀ=JCM 30384ᵀ).