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Lysobacter pocheonensis sp. nov., isolated from soil of a ginseng field

Siddiqi, Muhammad Zubair, Im, Wan-Taek
Archives of microbiology 2016 v.198 no.6 pp. 551-557
DNA, Lysobacter, Panax, bacteria, chemotaxonomy, fatty acid composition, fatty acids, nucleotide sequences, pH, ribosomal RNA, sequence analysis, soil, temperature, ubiquinones, South Korea
A Gram-staining-negative, non-spore-forming, non-flagellated, rod-shaped, catalase- and oxidase-negative bacterium, designated as Gsoil 193ᵀ, was isolated from the soil of ginseng field in Pocheon province, South Korea. 16S rRNA gene sequence analysis showed that strain Gsoil 193ᵀ belonged to family Xanthomonadaceae and was most closely related to Lysobacter daecheongensis KCTC 12,600ᵀ (96.4 %), Lysobacter panaciterrae KCTC 12601ᵀ (96.3 %), Lysobacter dokdonensis DSM 17958ᵀ (96.3 %) and Lysobacter oligotrophicus JCM 18257ᵀ (95.6 %). Strain Gsoil 193ᵀ grew at temperatures between 20 and 30 °C with an optimum of 30 °C. The pH range for growth was 5–9 pH (optimum 6–7 pH). The predominant respiratory quinone was ubiquinone Q-8 and a fatty acid profile with iso-C₁₅:₀, iso-C₁₆:₀ and summed feature 9 (iso-C₁₇:₁ ω9c/C₁₆:₀ 10-methyl) as the major fatty acids supported the affiliation of strain Gsoil 193ᵀ to the genus Lysobacter. The genomic DNA G+C content was 64.8 mol %. On the basis of the genotypic analysis, physiological and chemotaxonomic results indicate that strain Gsoil 193ᵀ represents a novel species of the genus Lysobacter, for which the name Lysobacter pocheonensis sp. nov. is proposed. The type strain is Gsoil 193ᵀ (= DSM 18338ᵀ = KCTC 12624ᵀ).