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Capillary zone electrophoresis for fatty acids with chemometrics for the determination of milk adulteration by whey addition

de Oliveira Mendes, Thiago, Porto, Brenda Lee Simas, Bell, Maria José Valenzuela, Perrone, Ítalo Tuler, de Oliveira, Marcone Augusto Leal
Food chemistry 2016 v.213 pp. 647-653
adulterated products, capillary zone electrophoresis, chemometrics, correlation, dairy industry, discriminant analysis, fatty acid composition, fatty acids, fraud, gas chromatography, milk, physicochemical properties, quality control, whey
Adulteration of milk with whey is difficult to detect because these two have similar physical and chemical characteristics. The traditional methodologies to monitor this fraud are based on the analysis of caseinomacropeptide. The present study proposes a new approach to detect and quantify this fraud using the fatty acid profiles of milk and whey. Fatty acids C14:0, C16:0, C18:0, C18:1, C18:2 and C18:3 were selected by gas chromatography associated with discriminant analysis to differentiate milk and whey, as they are present in quite different amounts. These six fatty acids were quantified within a short time by capillary zone electrophoresis in a set of adulterated milk samples. The correlation coefficient between the true values of whey addition and the experimental values obtained by this technique was 0.973. The technique is thus useful for the evaluation of milk adulteration with whey, contributing to the quality control of milk in the dairy industry.