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Clinical application of a ligation-independent pathway of multiplex ligation-dependent probe amplification for the determination of quinolone susceptibility of Streptococcus pneumoniae

Author:
Uno, Naoki, Araki, Nobuko, Kaku, Norihito, Kosai, Kousuke, Hasegawa, Hiroo, Yanagihara, Katsunori
Source:
Journal of microbiological methods 2016
ISSN:
0167-7012
Subject:
Streptococcus pneumoniae, antibiotic resistance, mutation, quinolones
Abstract:
We previously uncovered a ligation-independent pathway for multiplex ligation-dependent probe amplification (MLPA) through which products of MLPA could be amplified without both hybridization and ligation reactions. Here, we utilized this pathway to detect an antibiotic resistance mutation of quinolones in Streptococcus pneumoniae.
Agid:
5247597