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Molecular characterization and expression analysis of the large yellow croaker (Larimichthys crocea) complement component C6 after bacteria challenge

Liu, Wei, Jiang, Lihua, Dong, Xiangli, Liu, Xiaoxu, Kang, Lisen, Wu, Changwen
Aquaculture 2016 v.458 pp. 107-112
Larimichthys crocea, Vibrio anguillarum, amino acid sequences, bacteria, complement, complementary DNA, genes, immune response, innate immunity, kidneys, liver, open reading frames, quantitative polymerase chain reaction, spleen, tissues
The complement system is an indispensable component in innate immunity. As one of them, complement component C6 plays an important role in the complement system, mediating membrane attack complex (MAC) formation and bacterial lysis. In this study, full-length complement component C6 (LycC6) cDNA (GenBank accession no. KT962992) was cloned from the large yellow croaker (Larimichthys crocea) and an ORF of 3156bp was identified. The deduced amino acid sequence predicted that LycC6 contained conserved residues and domains known to be critical for C6 function. The quantitative real-time PCR analysis revealed that the LycC6 transcript was expressed in all the examined tissues. Complement component C3 is another central molecule in the complement system, converging the upstream complement signals and mediating the MAC assembly pathway formation. LycC6 was indispensable for active MAC formation after Vibrio anguillarum (V. anguillarum) challenge. Complement component C3 of large yellow croaker (LycC3) and LycC6 expression increased in the liver, spleen and kidney, and was the highest in the liver after V. anguillarum and polyinosinic:polycytidylic acid (Poly I:C) challenge. The results suggested that LycC6 might be an important immune-related gene, which is playing an important role in the immune defenses against bacterial infection.The complement component C6 is an important component that plays a major role in the complement system. This study provides the first investigation of the cloning and characterization of the complement component C6 gene from Larimichthys crocea, and analysis of the expression of complement component C6 and complement component C3 of Larimichthys crocea after Vibrio anguillarum and acid Poly I:C challenge. The work described has not been published or is under consideration for publication elsewhere. The study was reviewed and approved by the Ethics Committee of Animal Experiments of Zhejiang Ocean University.