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Gd (III) complex conjugate of low-molecular-weight chitosan as a contrast agent for magnetic resonance/fluorescence dual-modal imaging

Author:
Huang, Yan, Boamah, Peter Osei, Gong, Jianbo, Zhang, Qi, Hua, Mingqing, Ye, Yuzhen
Source:
Carbohydrate polymers 2016 v.143 pp. 288-295
ISSN:
0144-8617
Subject:
bovine serum albumin, chitosan, diagnostic sensitivity, fluorescein, fluorescence, gadolinium, hydrophobic bonding, image analysis
Abstract:
The fusion of molecular and anatomical modalities facilitates more reliable and accurate detection in clinic. In this work, we prepared gadolinium (III) complex Gd-DTPA-FITC-CS11 with magnetic resonance (MR) and fluorescence dual-modal imaging modalities. Gd-DTPA-FITC-CS11 consisted of fluorescein isothiocyanate and low-molecular-weight chitosan (CS11) units conjugated with gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). Gd-DTPA-FITC-CS11 exhibited a higher longitudinal relaxivity (14.09mM−1s−1) than the clinical Gd-DTPA (3.85mM−1s−1). T1-weighted MR contrast enhancement was also demonstrated the comparability to Gd-DTPA at lower dosage. The binding with bovine serum albumin (BSA) was investigated. The fluorescence of BSA in the presence of Gd-DTPA-FITC-CS11 was weakened due to static quenching mechanism. The conformation of BSA was slightly changed but α-helix was dominant. The binding was entropy-driven and spontaneous and the main contribution was hydrophobic interaction. Our results suggested the potential of Gd-DTPA-FITC-CS11 as an MR/fluorescence dual-modal imaging contrast agent in improving the diagnostic sensitivity and accuracy.
Agid:
5253385