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First detection of Leishmania kDNA in canine cerumen samples by qPCR

Belinchón-Lorenzo, Silvia, Parejo, Juan Carlos, Iniesta, Virginia, Fernández-Cotrina, Javier, Muñoz-Madrid, Rubén, Monroy, Isabel, Baz, Victoria, Gómez-Luque, Adela, Serrano-Aguilera, Francisco Javier, Barneto, José Luis, Gómez-Nieto, Luis Carlos
Veterinary parasitology 2016 v.228 pp. 65-68
Leishmania infantum, blood, blood sampling, bone marrow, dogs, kinetoplast DNA, lymph nodes, parasites, quantitative polymerase chain reaction, visceral leishmaniasis
Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be detected by Real time quantitative PCR (qPCR). A population of 38 dogs that lived in Leishmania endemic areas was divided in two groups: A (33 dogs with confirmed leishmaniosis by serological techniques) and B (5 healthy dogs). Blood, lymph node, bone marrow and cerumen samples from all animals were tested for the presence of parasite kDNA. Our method was 100% specific, and in dogs from group A, Leishmania infantum kDNA was detected and quantified in the 100% of lymph node samples, in 90.9% of cerumen samples, in 88.5% of the bone marrow samples and in 57.6% of the blood samples. The qPCR-cerumen is a new non-invasive method that shows a high potential for the diagnosis of zoonotic visceral leishmaniosis.