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Identification, characterization and description of Arcobacter faecis sp. nov., isolated from a human waste septic tank

Whiteduck-Léveillée, Kerri, Whiteduck-Léveillée, Jenni, Cloutier, Michel, Tambong, James T., Xu, Renlin, Topp, Edward, Arts, Michael T., Chao, Jerry, Adam, Zaky, Lévesque, C. André, Lapen, David R., Villemur, Richard, Khan, Izhar U.H.
Systematic and applied microbiology 2016 v.39 no.2 pp. 93-99
Arcobacter, fatty acid composition, humans, new species, nucleic acid hybridization, nucleotide sequences, phenotype, phylogeny, ribosomal RNA, septic systems, Ontario
A study on the taxonomic classification of Arcobacter species was performed on the cultures isolated from various fecal sources where an Arcobacter strain AF1078T from human waste septic tank near Ottawa, Ontario, Canada was characterized using a polyphasic approach. Genetic investigations including 16S rRNA, atpA, cpn60, gyrA, gyrB and rpoB gene sequences of strain AF1078T are unique in comparison with other arcobacters. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is most closely related to Arcobacter lanthieri and Arcobacter cibarius. Analyses of atpA, cpn60, gyrA, gyrB and rpoB gene sequences suggested that strain AF1078T formed a phylogenetic lineage independent of other species in the genus. Whole-genome sequence, DNA-DNA hybridization, fatty acid profile and phenotypic analysis further supported the conclusion that strain AF1078T represents a novel Arcobacter species, for which the name Arcobacter faecis sp. nov. is proposed, with type strain AF1078T (=LMG 28519T; CCUG 66484T).