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Development of a LAMP marker of the Puroindoline a-D1b allele in bread wheat
- Fukuta, Shiro, Tsuji, Takako, Suzuki, Ryoji, Matsumoto, Yuho, Ito, Kouji, Kataoka, Kouji, Kawara, Satomi, Miyake, Noriyuki
- Journal of cereal science 2016 v.69 pp. 264-266
- DNA, Triticum aestivum, alleles, genotype, hardness, loci, loop-mediated isothermal amplification, sodium hydroxide, texture, wheat, Japan
- Grain hardness of wheat (Triticum aestivum) is one of the most important characteristics for end-use properties. Grain texture is controlled by major locus Puroindoline gene. A large deletion of the majority of Puroindoline a (Pina) coding region cause hard texture. In this study, we report the development of a loop-mediated isothermal amplification (LAMP) reaction to identify Pina-D1b allele, which is popular genotype for hard type of bread wheat in Japan. In the LAMP method, Pina-D1b allele was easily detected using the simple NaOH extraction method and visualization of DNA amplification by hydroxynaphthol blue.