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Cannabis sativa and Humulus lupulus essential oils as novel control tools against the invasive mosquito Aedes albopictus and fresh water snail Physella acuta

Author:
Bedini, Stefano, Flamini, Guido, Cosci, Francesca, Ascrizzi, Roberta, Benelli, Giovanni, Conti, Barbara
Source:
Industrial crops and products 2016 v.85 pp. 318-323
ISSN:
0926-6690
Subject:
Aedes albopictus, Baetidae, Cannabis sativa, Humulus lupulus, Physidae, acute toxicity, antifeedants, beers, bitterness, bladder, brewing industry, chemical composition, essential oils, freshwater, gas chromatography-mass spectrometry, hemp, insects, invasive species, lethal concentration 50, mites, oilseeds, snails, terpenoids, vines
Abstract:
Over the past several decades, there has been a resurgence of interest in industrial hemp (Cannabis sativa L., Cannabaceae) cultivation. Besides fibre, seeds and oil, hemp contains high quantity of essential oil (EO). Hop (Humulus lupulus L., Cannabaceae) is a high-climbing, perennial vine, largely utilized in the brewing industry to add flavour and bitterness to beer. While it is known that hop also contains α- and ß-acids, and terpenes that have been found to be toxic, anti-feedant, and repellent for insects and mites, little is known about the bioactivity against problematic species of the hemp EO. In this study, the chemical composition of the EOs from C. sativa and H. lupulus was evaluated by GC–MS, and their acute toxicity was assessed against the Asian tiger mosquito Aedes albopictus (Skuse) (Diptera Culicidae) and, the freshwater bladder snail Physella acuta (Draparnaud) (Mollusca Physidae), two problematic invasive species. Furthermore, we evaluated the toxicity of both EOs against a non-target insect, the mayfly Cloeon dipterum L. (Ephemeroptera Baetidae). Both EOs were toxic against the three tested species. The most effective EO was the C. sativa, able to kill 100% of P. acuta snails starting from 100μLL−1. C. sativa LC50 were 301.560, 282.174 and, 35.370μLL−1, while H. lupulus LC50 were 330.855, 219.787 and, 118.653μLL−1 against A. albopictus, C. dipterum and P. acuta, respectively. Relative median potency analysis showed that the C. sativa EO was more toxic than H. lupulus against A. albopictus and P. acuta, while H. lupulus was more toxic than C. sativa EO against C. dipterum. The most susceptible species to the two EOs was P. acuta, while A. albopictus resulted the least susceptible one.
Agid:
5265243