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A DNA macro-array to simultaneously identify 32 meat species in food samples

Cottenet, Geoffrey, Sonnard, Véronique, Blancpain, Carine, Ho, Hui Zhen, Leong, Hann Lum, Chuah, Poh Fong
Food control 2016 v.67 pp. 135-143
DNA, adulterated products, analytical methods, cost effectiveness, fraud, laws and regulations, meat, polymerase chain reaction, taxonomic keys
Considering the recent cases of meat adulteration and fraud, efficient and accurate analytical methods are needed for the specific identification of meat species as a key importance to maintain consumers' trust and to comply with various local labelling legislations. As a reference approach, the application of PCR to meat speciation is always limited to few species, whereas there is a strong need to broaden the number of identified meat species. PCR was here associated with a commercial DNA macro-array spotted with meat-specific capture probes, allowing the simultaneous identification of 32 meat species on 8 samples per chip support. Tested on pure meat samples, spiked samples, proficiency test samples, and processed samples, the method showed high specificity on the targeted species and allowed a sensitivity down to 1% (w/w). Easy-to-use, fast and cost-efficient, this multi-screening approach is fit-for-purpose as a tool to detect meat substitution or adulteration for meat testing laboratories.