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A DNA macro-array to simultaneously identify 32 meat species in food samples

Author:
Cottenet, Geoffrey, Sonnard, Véronique, Blancpain, Carine, Ho, Hui Zhen, Leong, Hann Lum, Chuah, Poh Fong
Source:
Food control 2016 v.67 pp. 135-143
ISSN:
0956-7135
Subject:
DNA, adulterated products, analytical methods, cost effectiveness, fraud, laws and regulations, meat, polymerase chain reaction, taxonomic keys
Abstract:
Considering the recent cases of meat adulteration and fraud, efficient and accurate analytical methods are needed for the specific identification of meat species as a key importance to maintain consumers' trust and to comply with various local labelling legislations. As a reference approach, the application of PCR to meat speciation is always limited to few species, whereas there is a strong need to broaden the number of identified meat species. PCR was here associated with a commercial DNA macro-array spotted with meat-specific capture probes, allowing the simultaneous identification of 32 meat species on 8 samples per chip support. Tested on pure meat samples, spiked samples, proficiency test samples, and processed samples, the method showed high specificity on the targeted species and allowed a sensitivity down to 1% (w/w). Easy-to-use, fast and cost-efficient, this multi-screening approach is fit-for-purpose as a tool to detect meat substitution or adulteration for meat testing laboratories.
Agid:
5266254