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Bioflocculation of the oceanic microalga Dunaliella salina by the bloom-forming dinoflagellate Heterocapsa circularisquama, and its effect on biodiesel properties of the biomass

Cho, Kichul, Hur, Sung-Pyo, Lee, Chi-Heon, Ko, Kyungjun, Lee, Yeon-Ji, Kim, Kil-Nam, Kim, Moo-Sang, Chung, Young-Ho, Kim, Daekyung, Oda, Tatsuya
Bioresource technology 2016 v.202 pp. 257-261
Chlorella vulgaris, Dunaliella salina, Miozoa, Nannochloropsis, biodiesel, biomass, centrifugation, energy content, fatty acid methyl esters, flocculation, fluorescence, harvesting, iodine value, lipid content, microalgae
The aim of this work was to evaluate the flocculation by the dinoflagellate Heterocapsa circularisquama as a means for harvesting three Chlorophyta species, Chlorella vulgaris, Nannochloropsis granulata, and Dunaliella salina. Relative fluorescence of D. salina culture significantly decreased along with 9.3-fold increased flocculation activity within 24h when mixed with H. circularisquama. Lipid content of bioflocculated D. salina increased about 40%, while fatty acid methyl ester (FAME) profiles exhibited higher levels of C16:0, C18:0, and C18:1, compared to harvest by centrifugation, suggesting higher energy content. Furthermore, bioflocculated D. salina biomass had more suitable biodiesel properties relative to both EN14214 and ASTMD6751, with a cetane number of 49.0 and an iodine value of 95.9. These results suggest that H. circularisquama-induced bioflocculation is applicable for the sustainable and qualitative production of algal biodiesel.