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Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides
- Huang, Xiang-Feng, Liu, Jia-Nan, Lu, Li-Jun, Peng, Kai-Ming, Yang, Gao-Xiang, Liu, Jia
- Bioresource technology 2016 v.206 pp. 141-149
- Rhodosporidium toruloides, acetic acid, biodiesel, biomass production, carbon, carbon nitrogen ratio, fuel production, lipid content, raw materials, volatile fatty acids
- Rhodosporidium toruloides AS 2.1389 was tested using different concentrations of acetic acid as a low-cost carbon source for the production of microbial lipids, which are good raw materials for biodiesel production. It grew and had higher lipid contents in media containing 4–20g/L acetic acid as the sole carbon source, compared with that in glucose-containing media under the same culture conditions. At acetic acid concentrations as high as 20g/L and the optimal carbon-to-nitrogen ratio (C/N) of 200 in a batch culture, the highest biomass production was 4.35g/L, with a lipid content of 48.2%. At acetic acid concentrations as low as 4g/L, a sequencing batch culture (SBC) with a C/N of 100 increased biomass production to 4.21g/L, with a lipid content of 38.6%. These results provide usable culture strategies for lipid production by R. toruloides AS 2.1389 when using diverse waste-derived volatile fatty acids.