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Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides

Huang, Xiang-Feng, Liu, Jia-Nan, Lu, Li-Jun, Peng, Kai-Ming, Yang, Gao-Xiang, Liu, Jia
Bioresource technology 2016 v.206 pp. 141-149
Rhodosporidium toruloides, acetic acid, biodiesel, biomass production, carbon, carbon nitrogen ratio, fuel production, lipid content, raw materials, volatile fatty acids
Rhodosporidium toruloides AS 2.1389 was tested using different concentrations of acetic acid as a low-cost carbon source for the production of microbial lipids, which are good raw materials for biodiesel production. It grew and had higher lipid contents in media containing 4–20g/L acetic acid as the sole carbon source, compared with that in glucose-containing media under the same culture conditions. At acetic acid concentrations as high as 20g/L and the optimal carbon-to-nitrogen ratio (C/N) of 200 in a batch culture, the highest biomass production was 4.35g/L, with a lipid content of 48.2%. At acetic acid concentrations as low as 4g/L, a sequencing batch culture (SBC) with a C/N of 100 increased biomass production to 4.21g/L, with a lipid content of 38.6%. These results provide usable culture strategies for lipid production by R. toruloides AS 2.1389 when using diverse waste-derived volatile fatty acids.