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Cometabolic degradation of lincomycin in a Sequencing Batch Biofilm Reactor (SBBR) and its microbial community

Author:
Li, Yancheng, Zhou, Jian, Gong, Benzhou, Wang, Yingmu, He, Qiang
Source:
Bioresource technology 2016 v.214 pp. 589-595
ISSN:
0960-8524
Subject:
Fourier transform infrared spectroscopy, Halomonas, Roseovarius, Thiothrix, bacteria, biofilm, carbon, denaturing gradient gel electrophoresis, gas chromatography-mass spectrometry, glucose, lincomycin, microbial communities, redox potential, ribosomal DNA, wastewater
Abstract:
Cometabolism technology was employed to degrade lincomycin wastewater in Sequencing Batch Biofilm Reactor (SBBR). In contrast with the control group, the average removal rate of lincomycin increased by 56.0% and Total Organic Carbon (TOC) increased by 52.5% in the cometabolic system with glucose as growth substrate. Under the same condition, Oxidation–Reduction Potential (ORP) was 85.1±7.3mV in cometabolic system and 198.2±8.4mV in the control group, indicating that glucose changed the bulk ORP and created an appropriate growing environment for function bacteria. Functional groups of lincomycin were effectively degraded in cometabolic system proved by FTIR and GC–MS. Meanwhile, results of DGGE and 16S rDNA showed great difference in dominant populations between cometabolic system and the control group. In cometabolic system, Roseovarius (3.35%), Thiothrix (2.74%), Halomonas (2.49%), Ignavibacterium (2.02%), and TM7_genus_incertae_sedis (1.93%) were verified as dominant populations at genus level. Cometabolism may be synergistically caused by different functional dominant bacteria.
Agid:
5267423