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Jian carp (Cyprinus carpio var. Jian) intestinal immune responses, antioxidant status and tight junction protein mRNA expression are modulated via Nrf2 and PKC in response to dietary arginine deficiency

Author:
Wang, Biao, Feng, Lin, Chen, Gang-Fu, Jiang, Wei-Dan, Liu, Yang, Kuang, Sheng-Yao, Jiang, Jun, Tang, Ling, Wu, Pei, Tang, Wu-Neng, Zhang, Yong-An, Zhao, Juan, Zhou, Xiao-Qiu
Source:
Fish & shellfish immunology 2016 v.51 pp. 116-124
ISSN:
1050-4648
Subject:
Cyprinus carpio, antioxidants, arginine, carp, catalase, diet, gene expression, glutathione, glutathione peroxidase, glutathione transferase, glutathione-disulfide reductase, immune response, interleukin-1, interleukin-8, intestines, juveniles, malondialdehyde, messenger RNA, occludins, protein kinase C, superoxide dismutase, tight junctions, tumor necrosis factors, zinc
Abstract:
This study investigated the effect of dietary arginine on the immune response, antioxidant status and tight junction mRNA expression in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 1200 juvenile Jian carp with an average initial weight of 6.33 ± 0.03 g were fed graded levels of arginine (9.8–24.5 g kg−1 diet) for nine weeks. The study showed that arginine deficiency up-regulated interleukin 1, interleukin 8 and transforming growth factor-β and down-regulated tumour necrosis factor α gene expression (P < 0.05). Additionally, arginine deficiency increased malondialdehyde (MDA), protein carbonyl (PC) and glutathione contents and decreased the activities of copper/zinc superoxide dismutase (SOD1), glutathione peroxidase (GPx), catalase (CAT) and glutathione reductase (GR) and glutathione-S-transferase (GST) (P < 0.05). Meanwhile, arginine deficiency significantly increased claudin 7, occludin, protein kinase C, NF-E2-related factor 2 and Kelch-like-ECH- associated protein 1 mRNA expression and decreased SOD1, CAT and GR mRNA expression (P < 0.05). All of these results indicated that arginine deficiency impaired intestinal immune function via the regulation of mRNA expression of cytokines, tight junction proteins, antioxidant enzymes, Nrf2/Keap1 and PKC in fish intestine.
Agid:
5268731