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Immune responses to Aeromonas hydrophila infection in Schizothorax prenanti fed with oxidized konjac glucomannan and its acidolysis products

Author:
Zheng, Qiaoran, Wu, Yinglong, Xu, Huailiang, Wang, Hongjie, Tang, Haolan, Xia, Xiaojie, Feng, Jiao
Source:
Fish & shellfish immunology 2016 v.49 pp. 260-267
ISSN:
1050-4648
Subject:
Aeromonas hydrophila, Schizothorax prenanti, acidolysis, aquaculture, bacteria, bacterial infections, blood serum, complement, diet, feed additives, fish, gene expression, gene expression regulation, genes, immune response, interleukin-1beta, konjac mannan, lysozyme, oral administration, tumor necrosis factor-alpha
Abstract:
The objective of the present study was to determine the effect of dietary oxidized konjac glucomannan (OKGM) and its acidolysis products (L-OKGM) on the immune parameters and the gene expression profile of some inflammatory-related cytokines from Schizothorax prenanti during the early stages of injection with Aeromonas hydrophila. Fish were orally administered with seven different diets containing 0 g kg−1 (control diet), 8.0, 16.0 and 32.0 g kg−1 OKGM and L-OKGM diets for 60 days prior to injection. After 60 days, the control and the treated fish were intraperitoneally injected with 0.2 ml PBS or 2 × 107 cfu/ml bacteria per fish and sampled at time 6 h post-injection. The results showed that the serum lysozyme activity and complement C3 level of fish fed 8.0 g kg−1 L-OKGM was significantly increased after bacterial infection. Moreover, the injection with A. hydrophila generally up-regulated the expression of all measured genes when compared to their corresponding controls. When compared with the control group, the expression of TLR22, TNF-α and IL-1β was significantly increased in fish fed OKGM and L-OKGM diet after bacterial injection. Furthermore, the L-OKGM diet showed higher activity to trigger the immune response against bacteria, especially the low dosage L-OKGM diet. The results suggested that both of OKGM and L-OKGM are promising feed additive for S. prenanti in aquaculture.
Agid:
5268807