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Identification and mechanism of action of renoprotective constituents from peat moss Sphagnum palustre in cisplatin-induced nephrotoxicity

Kang, Hee Rae, Lee, Dahae, Eom, Hee Jeong, Lee, Seoung Rak, Lee, Kang Ro, Kang, Ki Sung, Kim, Ki Hyun
Journal of functional foods 2016 v.20 pp. 358-368
Sphagnum, apoptosis, betulinic acid, cisplatin, ergosterol, ethanol, fatty acids, flavonoids, kidney cells, mechanism of action, mitogen-activated protein kinase, natural resources, nephrotoxicity, phosphorylation, renoprotective effect
In search for bioactive constituents from natural resources, the ethanol (EtOH) extract of Sphagnum palustre showed significant renoprotective effects against cisplatin-induced damage in kidney cells. Phytochemical investigation of the EtOH extract led to the identification of a new flavonoid, 6-methyl-(2R,3S)-alpinone, along with six known flavonoids, four steroids, six triperpenoids, and three fatty acids. Among them, ergosterol peroxide, (3β,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid ameliorated cisplatin-induced nephrotoxicity to 80% of the control value at 125, 125 and 50 µM, respectively. Moreover, the elevated percentage of apoptotic cells by cisplatin was significantly reduced after co-treatment with the EtOH extract of S. palustre and ergosterol peroxide, (3β,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid. Upregulated phosphorylation of JNK and p38 by cisplatin treatment was markedly decreased after co-treatment with ergosterol peroxide, (3β,22E,24S)-3-hydroxy-ergosta-5,22-dien-7-one, and betulinic acid. These results show that blocking the MAPKs signalling cascade plays a critical role in mediating the renoprotective effect of S. palustre.