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C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice

Sun, Aning, Ren, Gaiyan, Deng, Chao, Zhang, Jingjing, Luo, Xiaoping, Wu, Xiaojun, Mani, Sridhar, Dou, Wei, Wang, Zhengtao
Journal of functional foods 2016 v.21 pp. 418-430
Toll-like receptor 4, colitis, dextran, flavonoids, fruits, genes, herbs, interleukin-1, mice, mitogen-activated protein kinase, myeloperoxidase, sodium sulfate, transcription factor NF-kappa B
Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-4 after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.