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Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1
- Harada, Gakuro, Pattarawat, Pawat, Ito, Kenji, Matsumoto, Takashi, Hasegawa, Takanori, Katakura, Yoshinori
- Journal of functional foods 2016 v.23 pp. 444-452
- Lactobacillus brevis, antineoplastic activity, cell senescence, cell viability, colorectal neoplasms, functional foods, genes, homeostasis, human cell lines, ingredients, lactic acid bacteria, neoplasm cells, novel foods, screening, telomerase, tissues
- SIRT1 is known to have critical functions in the maintenance of homeostasis and cell survival, and it confers anti-ageing effects on cells and tissues. To identify novel foods and food ingredients with potential anti-ageing functions, a novel system for screening substances that activate the SIRT1 promoter in human colorectal cancer cells was established. We screened several lactic acid bacteria (LAB) and identified Lactobacillus brevis T2102 as a SIRT1-activating strain of LAB. T2102 inactivated β-catenin through SIRT1-mediated deacetylation and consequently suppressed the growth of the human colorectal cancer cell line DLD-1. Furthermore, T2102-induced degradation of β-catenin repressed transcription of the human telomerase reverse transcriptase (hTERT) gene, one of the target genes of β-catenin, which led to the induction of cellular senescence and concomitant growth suppression of DLD-1. These results indicate that SIRT1-activating T2102 might be a promising candidate for developing novel functional foods with anti-cancer properties.