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Extract from spent hop (Humulus lupulus L.) reduces blood platelet aggregation and improves anticoagulant activity of human endothelial cells in vitro
- Luzak, Boguslawa, Golanski, Jacek, Przygodzki, Tomasz, Boncler, Magdalena, Sosnowska, Dorota, Oszmianski, Jan, Watala, Cezary, Rozalski, Marcin
- Journal of functional foods 2016 v.22 pp. 257-269
- Humulus lupulus, animal disease models, anticoagulant activity, blood platelets, coumaric acids, diabetes, endothelial cells, endothelium, flavanols, flavonols, glycosides, humans, placebos, platelet aggregation, proanthocyanidins, rats
- Spent hop extract (SHE) is hypothesized to possess multifaceted anticoagulant effect and to reduce platelet reactivity in a direct manner and to improve the antiplatelet action of endothelium. The results demonstrated that SHE, rich in flavanols (50% of total phenolic compounds), hydroxycinnamic acids, proanthocyanidin oligomers, flavan-3-ol monomers, and flavonol glycosides, inhibited ADP-induced platelet aggregation up to 11% for 7.5 µg/ml or up to 23% for 15 µg/ml, P < 0.05. Additionally, SHE ameliorated anticoagulant activity of human endothelial cells to significantly reduce platelet reactivity. This effect could be related to the increased expression/activity of the ecto-ADPase (CD39). ADP-induced platelet reactivity was reduced in the case of platelets incubated with the extract-treated endothelial cells compared to the untreated cells. Diabetic rats fed with SHE demonstrated longer survival time than the group treated with placebo. In conclusion, our study provided evidence for a potential beneficial role of SHE in the regulation of platelet and endothelial function.