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Molecular characterization of Vibrio harveyi bacteriophages isolated from aquaculture environments along the coast of India

Author:
Shivu, Mockshanath M., Rajeeva, Bettada C., Girisha, Shivani K., Karunasagar, Indrani, Krohne, Georg, Karunasagar, Iddya
Source:
Environmental microbiology 2007 v.9 no.2 pp. 322-331
ISSN:
1462-2912
Subject:
DNA, Myoviridae, Siphoviridae, Vibrio harveyi, aquaculture systems, bacteriophages, campylobacteriosis, coasts, coat proteins, cytolysis, hatcheries, host range, mariculture, polyacrylamide gel electrophoresis, random amplified polymorphic DNA technique, restriction fragment length polymorphism, shrimp, shrimp culture, streams, structural proteins, viral morphology, India
Abstract:
Seven bacteriophages specific to Vibrio harveyi, the causative agent of luminous vibriosis in shrimp, were isolated from coastal aquaculture systems like shrimp farms, hatcheries and tidal creeks along the east and west coast of India. All the seven phages were found to have the typical head and tail morphology with double-stranded DNA as genetic material. Morphologically, six phages were grouped under family Siphoviridae and one under Myoviridae. These phages were further characterized with respect to host range, morphology and structural proteins. Genomic fingerprinting was carried out using restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD). Major capsid proteins of all the phages detected by SDS-PAGE were distinct from one another. All the phages were found to be highly lytic for V. harveyi and had different lytic spectrum for the large number of isolates tested. Six of the seven phages isolated had a broad lytic spectrum and could be potential candidates for biocontrol of V. harveyi in aquaculture systems.
Agid:
527349