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Facile Fabrication of Gold Nanoparticles-Poly(vinyl alcohol) Electrospun Water-Stable Nanofibrous Mats: Efficient Substrate Materials for Biosensors

Author:
Wang, Juan, Yao, Hong-Bin, He, Dian, Zhang, Chuan-Ling, Yu, Shu-Hong
Source:
ACS Applied Materials & Interfaces 2012 v.4 no.4 pp. 1963-1971
ISSN:
1944-8252
Subject:
alcohols, biosensors, catalytic activity, crosslinking, electrostatic interactions, gold, hydrogen peroxide, nanofibers, nanogold, peroxidase, porosity, tensile strength, tissue engineering
Abstract:
Electrospun nanofibrous mats are intensively studied as efficient scaffold materials applied in the fields of tissue engineering, catalysis, and biosensors due to their flexibility and porosity. In this paper, we report a facile route to fabricate gold nanoparticles-poly(vinyl alcohol) (Au NPs-PVA) hybrid water stable nanofibrous mats with tunable densities of Au NPs and further demonstrate the potential application of as-prepared Au NPs-PVA nanofibrous mats as efficient biosensor substrate materials. First, through the designed in situ cross-linkage in coelectrospun PVA-glutaraldehyde nanofibers, water insoluble PVA nanofibrous mats with suitable tensile strength were successfully prepared. Then, 3-mercaptopropyltrimethoxysilane (MPTES) was modified on the surface of obtained PVA nanofibrous films, which triggered successful homogeneous decoration of Au NPs through gold–sulfur bonding interactions. Finally, the Au NPs-PVA nanofibrous mats embedded with horseradish peroxidase (HRP) by electrostatic interactions were used as biosensor substrate materials for H₂O₂ detection. The fabricated HRP-Au NPs/PVA biosensor showed a highly sensitive detection of H₂O₂ with a detection limit of 0.5 μM at a signal-to-noise ratio of 3. By modifying other different functional nanaoparticles or enzyme on the PVA nanofibrous film will further expand their potential applications as substrate materials of different biosensors.
Agid:
5273951