PubAg

Main content area

Analysis of Two Gene Clusters Involved in the Degradation of 4-Fluorophenol by Arthrobacter sp. Strain IF1

Author:
Ferreira, Maria Isabel M., Iida, Toshiya, Hasan, Syed A., Nakamura, Kaoru, Fraaije, Marco W., Janssen, Dick B., Kudo, Toshiaki
Source:
Applied and environmental microbiology 2009 v.75 no.24 pp. 7767-7773
ISSN:
0099-2240
Subject:
Arthrobacter, DNA libraries, DNA primers, Escherichia coli, NAD (coenzyme), Northern blotting, adenine, carbon, clones, dehalogenation, energy, gene expression, hydroquinone, hydroxylation, multigene family, p-nitrophenol, reverse transcriptase polymerase chain reaction, screening
Abstract:
Arthrobacter sp. strain IF1 is able to grow on 4-fluorophenol (4-FP) as a sole source of carbon and energy. To clone the 4-FP degradation genes, DNA libraries were constructed and screened with a probe obtained by PCR using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. Sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4-chlorophenol monooxygenase systems. Both these monooxygenase genes were differentially expressed during growth on 4-FP, as revealed by Northern blotting and reverse transcription-PCR. One cluster also contained a gene for a flavin reductase. The monooxygenase and reductase were purified from Escherichia coli cells expressing the corresponding genes, and together they catalyzed NADH-dependent hydroxylation and dehalogenation of 4-halophenols. The results indicate that strain IF1 transforms 4-FP to hydroquinone by a two-component monooxygenase system of which one component provides reduced flavin adenine dinucleotide at the expense of NADH and the other catalyzes para-hydroxylation of 4-FP and other 4-substituted phenols.
Agid:
527644