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Visualization of Zn2+ Ions in Live Zebrafish Using a Luminescent Iridium(III) Chemosensor
- Ma, Dik-Lung, He, Hong-Zhang, Zhong, Hai-Jing, Lin, Sheng, Chan, Daniel Shiu-Hin, Wang, Liang, Lee, Simon
Ming-Yuen, Leung, Chung-Hang, Wong, Chun-Yuen
- ACS applied materials 2014 v.6 no.16 pp. 14008-14015
- Danio rerio, cadmium, calcium, cobalt, color, copper, electron transfer, ions, iridium, iron, lead, ligands, luminescence, magnesium, manganese, mercury, metal ions, nickel, nuclear magnetic resonance spectroscopy, potassium, silver, sodium, titration, ultraviolet-visible spectroscopy, zinc
- A novel luminescent cyclometalated iridium(III) complex-based chemosensor (1) bearing a zinc-specific receptor, tris(2-pyridylmethyl)amine, and the 3-phenyl-1H-pyrazole ligand has been designed and synthesized. Upon the addition of Zn²⁺ ions to a solution of iridium(III) complex 1, a pronounced luminescence color change from blue to green can be observed, which may be attributed to the suppression of photoinduced electron transfer upon complexation of complex 1 with Zn²⁺ ions. The interaction of iridium(III) complex 1 with Zn²⁺ ions was investigated by UV–vis absorption titration, emission titration, and ¹H NMR titration. Furthermore, the iridium(III) complex 1 exhibited good selectivity for Zn²⁺ over 13 other common metal ions, including K⁺, Ag⁺, Na⁺, Ni²⁺, Fe³⁺, Hg²⁺, Cd²⁺, Mg²⁺, Ca²⁺, Cu²⁺, Mn²⁺, Co²⁺, and Pb²⁺ ions. The practical application of the iridium(III) complex 1 in visualizing intracellular Zn²⁺ distribution in live zebrafish was also demonstrated.