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Polythiophene-g-poly(ethylene glycol) with Lateral Amino Groups as a Novel Matrix for Biosensor Construction

Author:
Akbulut, Huseyin, Bozokalfa, Guliz, Asker, Duygu N., Demir, Bilal, Guler, Emine, Odaci Demirkol, Dilek, Timur, Suna, Yagci, Yusuf
Source:
ACS Applied Materials & Interfaces 2015 v.7 no.37 pp. 20612-20622
ISSN:
1944-8252
Subject:
atomic force microscopy, biofilm, biosensors, composite polymers, dielectric spectroscopy, engineering, fluorescence emission spectroscopy, fluorescence microscopy, gel chromatography, laccase, models, nuclear magnetic resonance spectroscopy, pH, phenolic compounds, polymerization, scanning electron microscopy, ultraviolet-visible spectroscopy, wastewater
Abstract:
In the ever-expanding field of conducting polymer research, functionalized graft hybrid copolymers have gained considerable interest in the biomedical engineering and biosensing applications, particularly. In the present work, a new biosensor based on conducting graft copolymer for the detection of phenolic compounds was developed. Thereby, a robust and novel material, namely “polythiophene-g-poly(ethylene glycol) with lateral amino groups” (PT-NH₂-g-PEG) hybrid conducting polymer was synthesized via Suzuki condensation polymerization and characterized with ¹H NMR analysis, UV–vis spectroscopy, gel permeation chromatography (GPC) and fluorescence spectroscopy. PT-NH₂-g-PEG architecture was then applied as an immobilization matrix to accomplish extended biosensing function. In a typical process, Laccase was utilized as a model enzyme for the detection of phenolic compounds. Detailed surface characterization of PT-NH₂-g-PEG/Lac was performed by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy, fluorescence microscopy and scanning electron microscopy measurements. Optimum pH and polymer amount were found to be pH 6.5 and 0.5 mg polymer, respectively, with the linear range of 0.0025–0.05 mM and 132.45 μA/mM sensitivity. The kinetic parameters of PT-NH₂-g-PEG/Lac are 0.026 mM for Kₘᵃᵖᵖ and 7.38 μA for Iₘₐₓ, respectively. Furthermore, the PT-NH₂-g-PEG/Lac biofilm was retained 82% of its activity for 12 days indicating excellent recovery as tested with artificial wastewater.
Agid:
5284216