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Facile Synthesis of Biocompatible Fluorescent Nanoparticles for Cellular Imaging and Targeted Detection of Cancer Cells
- Tang, Fu, Wang, Chun, Wang, Xiaoyu, Li, Lidong
- ACS Applied Materials & Interfaces 2015 v.7 no.45 pp. 25077-25083
- biocompatibility, biopolymers, fluorescence, fluorescent dyes, folate receptors, folic acid, image analysis, nanoparticles, neoplasm cells, neoplasms, silver
- In this work, we report the facile synthesis of functional core–shell structured nanoparticles with fluorescence enhancement, which show specific targeting of cancer cells. Biopolymer poly-l-lysine was used to coat the silver core with various shell thicknesses. Then, the nanoparticles were functionalized with folic acid as a targeting agent for folic acid receptor. The metal-enhanced fluorescence effect was observed when the fluorophore (5-(and-6)-carboxyfluorescein-succinimidyl ester) was conjugated to the modified nanoparticle surface. Cellular imaging assay of the nanoparticles in folic acid receptor-positive cancer cells showed their excellent biocompatibility and selectivity. The as-prepared functional nanoparticles demonstrate the efficiency of the metal-enhanced fluorescence effect and provide an alternative approach for the cellular imaging and targeting of cancer cells.