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Fabrication of High-Performance Magnetic Lysozyme-Imprinted Microsphere and Its NIR-Responsive Controlled Release Property

Chen, Jinxing, Lei, Shan, Xie, Yunyun, Wang, Mozhen, Yang, Jun, Ge, Xuewu
ACS Applied Materials & Interfaces 2015 v.7 no.51 pp. 28606-28615
Escherichia coli, adsorption, bioactive properties, chemical elements, egg albumen, iron oxides, lysozyme, magnetic materials, nanoparticles, polymers, silica, surface area, thermodynamics
The preparation of efficient and practical biomacromolecules imprinted polymer materials is still a challenging task because of the spatial hindrance caused by the large size of template and target molecules in the imprinting and recognition process. Herein, we provided a novel pathway to coat a NIR-light responsive lysozyme-imprinted polydopamine (PDA) layer on a fibrous SiO₂ (F-SiO₂) microsphere grown up from a magnetic Fe₃O₄ core nanoparticle. The magnetic core–shell structured lysozyme-imprinted Fe₃O₄@F-SiO₂@PDA microspheres (MIP-lysozyme) can be easily separated by a magnet and have a high saturation adsorption capacity of lysozyme of 700 mg/g within 30 min because of the high surface area of 570 m²/g and the mesopore size of 12 nm of the Fe₃O₄@F-SiO₂ support. The MIP-lysozyme microspheres also show an excellent selective adsorption of lysozyme (IF > 4). The binding thermodynamic parameters studied by ITC proves that the lysozyme should be restricted by the well-defined 3D structure of MIP-lysozyme microspheres. The MIP-lysozyme can extract lysozyme efficiently from real egg white. Owing to the efficient NIR light photothermal effect of PDA layer, the MIP-lysozyme microspheres show the controlled release property triggered by NIR laser. The released lysozyme molecules still maintain good bioactivity, which can efficiently decompose E. coli. Therefore, this work provides a novel strategy to build practical NIR-light-responsive MIPs for the extraction and application of biomacromolecules.