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Facile Preparation of Biocompatible and Robust Fluorescent Polymeric Nanoparticles via PEGylation and Cross-Linking
- Li, Haiyin, Zhang, Xiqi, Zhang, Xiaoyong, Wang, Ke, Liu, Hongliang, Wei, Yen
- ACS Applied Materials & Interfaces 2015 v.7 no.7 pp. 4241-4246
- biocompatibility, chemical bonding, composite polymers, crosslinking, dispersibility, ethylene glycol, fluorescence, fluorescent dyes, image analysis, micelles, nanoparticles, polyethylene glycol, polymerization
- Novel cross-linked copolymers of PEG-IM-PhNH₂ are successfully synthesized through PEGylation via radical polymerization of 2-isocyanatoethyl methacrylate and poly(ethylene glycol) monomethyl ether methacylate and subsequent cross-linking with an amino-terminated aggregation-induced emission fluorogen. Such obtained amphiphilic copolymers can self-assemble to form uniform fluorescent polymeric nanoparticles (FPNs) and be utilized for cell imaging. These cross-linked FPNs are demonstrated good water dispersibility with ultralow critical micelle concentration (∼0.002 mg mL–¹), uniform morphology (98 ± 2 nm), high red fluorescence quantum yield, and excellent biocompatibility. More importantly, this novel strategy of fabricating cross-linked FPNs paves the way to the future development of more robust and biocompatible fluorescent bioprobes.