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Assembly of DNA Curtains Using Hydrogen Silsesquioxane As a Barrier to Lipid Diffusion

Author:
Fazio, T. A., Lee, Ja Yil, Wind, S. J., Greene, E. C.
Source:
Analytical chemistry 2012 v.84 no.18 pp. 7613-7617
ISSN:
1520-6882
Subject:
DNA, DNA assembly, hydrogen, image analysis, lipid bilayers, lipids, manufacturing, silica
Abstract:
We have established a single-molecule imaging experimental platform called “DNA curtains” in which DNA molecules tethered to a lipid bilayer are organized into patterns at nanofabricated metallic barriers on the surface of a microfluidic sample chamber. This technology has wide applications for real-time single-molecule imaging of protein–nucleic acid interactions. Here, we demonstrate that DNA curtains can also be made from hydrogen silsesquioxane (HSQ). HSQ offers important advantages over metallic barriers because it can be lithographically patterned directly onto fused silica slides without any requirement for further processing steps, thereby offering the potential for rapid prototype development and/or scale up for manufacturing.
Agid:
5291059