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High-Resolution Differential Ion Mobility Spectrometry of a Protein
- Shvartsburg, Alexandre A., Smith, Richard D.
- Analytical chemistry 2013 v.85 no.1 pp. 10-13
- electric field, gases, proteomics, spectroscopy, ubiquitin
- Use of elevated electric fields and helium-rich gases has recently enabled differential ion mobility spectrometry (IMS) with a resolving power up to R ∼ 300. Here we applied that technique to a protein (ubiquitin), achieving R up to ∼80 and separating previously unresolved conformers. While still limited by conformational multiplicity, this resolution is some 4 times greater than that previously reported using either conventional (drift-tube or traveling-wave) or differential IMS. The capability for fine resolution of protein conformers may open new avenues for proteoform separations in top-down and intact-protein proteomics.