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Structural characterization and anti-tumor effects of an inulin-type fructan from Atractylodes chinensis
- Xu, Jing, Chen, Dan, Liu, Chang, Wu, Xiong-Zhi, Dong, Cai-Xia, Zhou, Jing
- International journal of biological macromolecules 2016 v.82 pp. 765-771
- Atractylodes lancea, Fourier transform infrared spectroscopy, animal ovaries, antineoplastic activity, cell proliferation, derivatization, fructans, fructose, gas chromatography-mass spectrometry, glucose, hepatoma, high performance liquid chromatography, human cell lines, liver, molecular weight, neoplasm cells, nuclear magnetic resonance spectroscopy, ovarian neoplasms, polymerization, rhizomes, uterine cervical neoplasms
- A fructan (ACPS-1) with a molecular weight of 11.2kDa was isolated from Atractylodes chinensis rhizome and characterized by chemical derivatization, HPLC, GC–MS, FT-IR, and NMR. Structural analyses revealed that ACPS-1 is predominately composed of fructose and a small amount of glucose and a polymerization degree of about 53. The fructan was deduced to be an inulin-type fructan containing a linear backbone composed of (2→1)-linked β-d-Fruf residues. The in vitro antitumor activity of ACPS-1 was evaluated on four human cancer cell lines, including a cervical cancer cell line (Hela), two liver hepatocellular carcinoma cell lines (HepG2 and 7721), and an ovarian carcinoma cell line (Skov3). Results showed that ACPS-1 could significantly inhibit Hela, HepG2, and 7721 cell proliferation, especially HepG2, for which the fructan showed a proliferative inhibition rate as high as 87.40%. This result suggests that ACPS-1 may have anticancer potentiality against hepatocellular carcinoma and warrants further investigation.