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Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents

Tummalapalli, Mythili, Berthet, Morgane, Verrier, Bernard, Deopura, B.L., Alam, M.S., Gupta, Bhuvanesh
International journal of biological macromolecules 2016 v.82 pp. 104-113
Aloe vera, angiogenesis, animal disease models, anti-infective properties, anti-inflammatory activity, apoptosis, biocomposites, collagen, cotton fabric, curcumin, drugs, fibroblasts, finishes, gelatin, histology, leaching, mice, nonwoven fabrics, pectins, plant extracts, tissue repair, viability, wound treatment
Aloe vera and curcumin loaded oxidized pectin–gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism – erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.