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Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells

Author:
Li, Chao, Huang, Qiang, Xiao, Jie, Fu, Xiong, You, Lijun, Liu, Rui Hai
Source:
International journal of biological macromolecules 2016 v.91 pp. 671-679
ISSN:
0141-8130
Subject:
Prunella vulgaris, antioxidants, apoptosis, caspase-3, cell cycle checkpoints, chromatin, functional foods, hepatoma, human cell lines, humans, immunomodulation, interphase, mitochondria, neoplasm cells, polysaccharides, reactive oxygen species
Abstract:
Prunella vulgaris polysaccharides have been reported to have antioxidant, antitumor and immunomodulatory activities. In this study, P. vulgaris polysaccharide (P1)-zinc complex (P1-Zn) was first prepared by a facile method and its antiproliferative effect on HepG2 human hepatocellular carcinoma cells was also investigated. Results showed that P1-Zn could effectively inhibit the proliferation (98.4% inhibition rate at 500μg/mL) of HepG2 cells through induction of apoptosis, evidenced by morphological changes, chromatin condensation and G0/G1 phase cell cycle arrest. The intracellular mechanism of P1-Zn induced apoptosis was found to be the involvement of the activation of caspase-3 and -9, reactive oxygen species (ROS) overproduction and mitochondrial dysfunction. Our findings suggest that P1-Zn may be a potent candidate for human hepatocellular carcinoma treatment and prevention in functional foods and pharmacological fields.
Agid:
5294378