Main content area

Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells

Li, Chao, Huang, Qiang, Xiao, Jie, Fu, Xiong, You, Lijun, Liu, Rui Hai
International journal of biological macromolecules 2016 v.91 pp. 671-679
Prunella vulgaris, antioxidants, apoptosis, caspase-3, cell cycle checkpoints, chromatin, functional foods, hepatoma, human cell lines, humans, immunomodulation, interphase, mitochondria, neoplasm cells, polysaccharides, reactive oxygen species
Prunella vulgaris polysaccharides have been reported to have antioxidant, antitumor and immunomodulatory activities. In this study, P. vulgaris polysaccharide (P1)-zinc complex (P1-Zn) was first prepared by a facile method and its antiproliferative effect on HepG2 human hepatocellular carcinoma cells was also investigated. Results showed that P1-Zn could effectively inhibit the proliferation (98.4% inhibition rate at 500μg/mL) of HepG2 cells through induction of apoptosis, evidenced by morphological changes, chromatin condensation and G0/G1 phase cell cycle arrest. The intracellular mechanism of P1-Zn induced apoptosis was found to be the involvement of the activation of caspase-3 and -9, reactive oxygen species (ROS) overproduction and mitochondrial dysfunction. Our findings suggest that P1-Zn may be a potent candidate for human hepatocellular carcinoma treatment and prevention in functional foods and pharmacological fields.