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Polysaccharides from Arctium lappa L.: Chemical structure and biological activity

Author:
Carlotto, Juliane, de Souza, Lauro M., Baggio, Cristiane H., Werner, Maria Fernanda de P., Maria-Ferreira, Daniele, Sassaki, Guilherme L., Iacomini, Marcello, Cipriani, Thales R.
Source:
International journal of biological macromolecules 2016 v.91 pp. 954-960
ISSN:
0141-8130
Subject:
Arctium lappa, arabinogalactans, arabinose, bioactive properties, chemical structure, dose response, edema, fractionation, galactose, galacturonic acid, glucose, leaves, mannose, methylation, nuclear magnetic resonance spectroscopy, rhamnose, ultrafiltration
Abstract:
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.
Agid:
5294399