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Polysaccharides from Arctium lappa L.: Chemical structure and biological activity

Carlotto, Juliane, de Souza, Lauro M., Baggio, Cristiane H., Werner, Maria Fernanda de P., Maria-Ferreira, Daniele, Sassaki, Guilherme L., Iacomini, Marcello, Cipriani, Thales R.
International journal of biological macromolecules 2016 v.91 pp. 954-960
Arctium lappa, arabinogalactans, arabinose, bioactive properties, chemical structure, dose response, edema, fractionation, galactose, galacturonic acid, glucose, leaves, mannose, methylation, nuclear magnetic resonance spectroscopy, rhamnose, ultrafiltration
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.