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Isolation and characterization of eighteen polymorphic microsatellite markers from the frog flounder, Pleuronichthys cornutus

Author:
Che, Dongsheng, Jiang, Jiajun, Wang, Xubo
Source:
Conservation genetics resources 2013 v.5 no.2 pp. 335-337
ISSN:
1877-7252
Subject:
flounder, genomic libraries, loci, microsatellite repeats
Abstract:
This paper reports 18 polymorphic microsatellite markers from a severely over exploited and endangered flat fish, frog flounder (Pleuronichthys cornutus). The primers were designed from a genomic library enriched with dinucleotide (AC) repeats and screened on 30 samples from a natural population. The average allele number was 10.22 per locus (range 6–14) while the observed and expected heterozygosities ranging from 0.3846 to 1.0000 and 0.6810 to 0.9286, respectively. These polymorphic microsatellite loci would be applied on population genetic studies of P. cornutus.
Agid:
529653