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Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages

Author:
Gao, Peng, Yuan, Ming, Ma, Xianwei, Jiang, Wei, Zhu, Lingxi, Wen, Mingyue, Xu, Jing, Liu, Qiuyan, An, Huazhang
Source:
Molecular Immunology 2016 v.71 pp. 184-191
ISSN:
0161-5890
Subject:
chromatin, genes, immune response, macrophages, precipitin tests, transcription (genetics), transcription factors
Abstract:
IL-27 is an important regulator of TLR4-activated innate immune. The mechanism by which IL-27 production is regulated in TLR4-activated innate immune remains largely unclear. Here we show that expression of transcription factor Fli-1 at protein level is increased in macrophages following LPS stimulation. Fli-1 overexpression increases LPS-activated IL-27 production in macrophages. Consistently, Fli-1 knockdown inhibits LPS-induced IL-27 production in macrophages. Chromatin immunoprecipitation (ChIP) assay reveals that Fli-1 binds the promoter of IL-27 p28 subunit. Further experiments manifest that Fli-1 binds the region between −250 and −150bp upstream of the transcriptional start site of p28 gene and increases p28 gene promoter-controlled transcription. These results demonstrate that Fli-1 positively regulates IL-27 production in TLR4-activated immune response by promoting transcription of IL-27 p28 gene.
Agid:
5296877